Overview
Type: Synthetic peptide
Form: Lyophilized powder
ASIC1 Blocking Peptide (#BLP-SC014) is the original antigen used for immunization during Anti-ASIC1 Antibody (#ASC-014) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-ASIC1 Antibody (#ASC-014), (1:200).
2. Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Expression of ASIC1 in rat brainImmunohistochemical staining of rat globus pallidus using Anti-ASIC1 Antibody (#ASC-014). A. Parvalbumin (PV) positive neurons are shown (green). B. Neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (red). C. Double labeling with mouse anti-Parvalbumin (green) reveals strong co-localization of PV with ASIC1.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).
3,4. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).
2. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Multiplex staining of ASIC1 and parvalbumin in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:300). A. ASIC1 (green) is detected in the hilus of the dentate gyrus (arrows). B. The same section was stained with anti-parvalbumin (red). C. Merge of the two images shows partial colocalization (orange, see arrows). Cell nuclei in the same section were stained with DAPI (Blue).
Properties
Sequence
- CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926).
Accession (Uniprot) Number P55926
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
