Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926).
Accession (Uniprot) Number P55926
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-ASIC1 Antibody (#ASC-014), (1:200).
2. Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).- Rat brain sections.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).
3,4. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).
2. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).- Rat brain sections (1:300). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
