Cat #: ESP-201_PACK
Size: 11 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-ASIC3 Antibody Cat #: ASC-018
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.
Guinea pig Anti-ASIC3 Antibody Cat #: ASC-018-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.
Anti-pan ASIC (extracellular) Antibody Cat #: ASC-031
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1 (Accession P55926), amino acid residues 367–381 of rat ASIC2 (Accession Q62962), amino acid residues 375–388 of rat ASIC3 (Accession O35240) and amino acid residues 378–390 of rat ASIC4 (Accession Q9JHS6). Extracellular loop.
Homology
ASIC1: Mouse, rat, human – identical.
ASIC2: Mouse, rat, human – 14/15 amino acid residues identical.
ASIC3: Mouse, rat, human – 12/15 amino acid residues identical.
ASIC4: Mouse, rat – 12/15 amino acid residues identical; human – 11/15 amino acid residues identical.
ASIC2: Mouse, rat, human – 14/15 amino acid residues identical.
ASIC3: Mouse, rat, human – 12/15 amino acid residues identical.
ASIC4: Mouse, rat – 12/15 amino acid residues identical; human – 11/15 amino acid residues identical.
Pharmacological Reagents
Cono AIVGRPRF-amide Cat #: STC-174
Size:
0.5 mg
MW: 914 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
50 µM.
Activity
Cono AIVGRPRF-amide is an ASIC3 channel positive modulator.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Cono RPRF-amide Cat #: STC-170
Size:
0.5 mg
MW: 574 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
50 µM.
Activity
Cono RPRF-amide is an ASIC3 channel positive modulator1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Cono VGRPRF-amide Cat #: STC-172
Size:
0.5 mg
MW: 730 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
50 µM.
Activity
Cono VGRPRF-amide is an ASIC3 channel positive modulator1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
GMQ hydrochloride Cat #: G-165
Size:
50 mg
Source: Synthetic
MW: 237.1
Purity: >99%
Effective concentration
1-5 mM.
Activity
GMQ is an ASIC modulator, an ASIC3 opener and also induces an acidic shift of the pH dependence of inactivation of ASIC1a, -1b, -2a, and -31.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
APETx2 Cat #: STA-160
Size:
0.1 mg
MW: 4561 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10 nM - 3 µM.
Activity
APETx2 inhibits ASIC3 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#ASC-018-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
ASIC3 Channel Overexpressed Membrane Fractions includes:
1 x 0.1 ml lyophilized ASIC3 channel overexpressed membrane fractions
1 x 0.1 ml lyophilized non-injected Xenopus oocyte membrane fractions
For research purposes only, not for human use
