Overview
Type: Synthetic peptide
Form: Lyophilized powder
ASIC3 Blocking Peptide (#BLP-SC018) is the original antigen used for immunization during Anti-ASIC3 Antibody (#ASC-018) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysate:1. Anti-ASIC3 Antibody (#ASC-018), (1:200).
2. Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
Multiplex staining of ASIC1 and ASIC3 in rat DRGImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).- Rat trigeminal ganglia (1:400) (Yan, J. et al. (2013) Headache 53, 1250.).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP), (1:1000).
3,4. Guinea pig Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
Western blot analysis of rat dorsal root ganglion (DRG) lysate:1. Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP), (1:1000).
2. Guinea pig Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
Expression of ASIC3 in mouse dorsal raphe nucleusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP), (1:200), followed by goat-anti-guinea pig-AlexaFluor-488 secondary antibody. ASIC3 staining (green) appears in the soma of dorsal raphe nucleus (DRN) neurons (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240).
Accession (Uniprot) Number O35240
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
