Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240).
Accession (Uniprot) Number O35240
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysate:1. Anti-ASIC3 Antibody (#ASC-018), (1:200).
2. Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).- Rat trigeminal ganglia (1:400) (Yan, J. et al. (2013) Headache 53, 1250.).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP), (1:1000).
3,4. Guinea pig Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
Western blot analysis of rat dorsal root ganglion (DRG) lysate:1. Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP), (1:1000).
2. Guinea pig Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).- Mouse brain sections (1:200).
Last update: 02/01/2024
