Cat #: BLP-SC015
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- CKIKFAEEDAKPKEKEAGDE, corresponding to amino acid residues 7-26 of rat ASIC4 (Accession Q9QYV9).
Accession (Uniprot) Number Q9QYV9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse ND7/23 neuroblastoma x rat DRG neuron hybrid cell line (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates:1,2. Anti-ASIC4 Antibody (#ASC-015), (1:200).
3,4. Anti-ASIC4 Antibody, preincubated with ASIC4 Blocking Peptide (#BLP-SC015). - Western blot analysis of rat DRG lysates:1. Anti-ASIC4 Antibody (#ASC-015), (1:200).
2. Anti-ASIC4 Antibody, preincubated with ASIC4 Blocking Peptide (#BLP-SC015).
Last update: 02/01/2024