Overview
Type: Synthetic peptide
Form: Lyophilized powder
ASIC5 (extracellular) Blocking Peptide (#BLP-SC032) is the original antigen used for immunization during Anti-ASIC5 (extracellular) Antibody (#ASC-032) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat small intestine (lanes 3 and 6) lysates:1-3. Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200).
4-6. Anti-ASIC5 (extracellular) Antibody, preincubated with ASIC5 (extracellular) Blocking Peptide (#BLP-SC032).
Expression of ASIC5 in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASIC5 immunoreactivity (green) is detected in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5 (Accession Q9R0Y1).
Accession (Uniprot) Number Q9R0Y1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
