Overview
Type: Synthetic peptide
Form: Lyophilized powder
ASTN1 (extracellular) Blocking Peptide (#BLP-NR151) is the original antigen used for immunization during Anti-ASTN1 (extracellular) Antibody (#ANR-151) generation. The blocking peptide binds and ‘blocks’ Anti-ASTN1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200).
3,4. Anti-ASTN1 (extracellular) Antibody, preincubated with ASTN1 (extracellular) Blocking Peptide (#BLP-NR151).
Expression of Astrotactin-1 in mouse hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Astrotactin-1 immunoreactivity (green) is observed in neurons (arrows) of hippocampal CA3 pyramidal layer (P). B. Astrotactin-1 staining (green) appears in neurons of cortex (arrows).
Properties
Sequence
- (C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1 (Accession Q61137).
Accession (Uniprot) Number Q61137
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
