Overview
Type: Synthetic peptide
Form: Lyophilized Powder
B1 Bradykinin Receptor (BDKRB1) (extracellular) Blocking Peptide (#BLP-BR013) is the original antigen used for immunization during Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody (#ABR-013) generation. The blocking peptide binds and ‘blocks’ Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat heart lysate (lanes 3 and 6):1-3. Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody (#ABR-013), (1:500).
4-6. Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody, preincubated with B1 Bradykinin Receptor (BDKRB1) (extracellular) Blocking Peptide (BLP-BR013).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 5), mouse J774 macrophage cell line lysate (lanes 2 and 6), mouse M1 myeloid leukemia cell line lysate (lanes 3 and 7) and mouse Neuro-2a neuroblastoma cell line lysate (lanes 4 and 8):1-4. Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody (#ABR-013), (1:400).
5-8. Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody, preincubated with B1 Bradykinin Receptor (BDKRB1) (extracellular) Blocking Peptide (BLP-BR013).
Properties
Sequence
- Peptide (C)HEAWHFVRMVELN, corresponding to amino acid residues 202 - 214 of mouse BDKRB1 (Accession Q61125).
Accession (Uniprot) Number Q61125
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
