Overview
Type: Synthetic peptide
Form: Lyophilized powder
B1 Bradykinin Receptor/BDKRB1 Blocking Peptide (#BLP-BR011) is the original antigen used for immunization during Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011) generation. The blocking peptide binds and ‘blocks’ Anti-B1 Bradykinin Receptor/BDKRB1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and heart (lanes 2 and 4) lysates:1-2. Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011), (1:200).
3-4. Anti-B1 Bradykinin Receptor (BDKRB1) Antibody, preincubated with B1 Bradykinin Receptor/BDKRB1 Blocking Peptide (#BLP-BR011).
Expression of B1 bradykinin receptor in rat CNSImmunohistochemical staining of frozen rat brain section (A & B) and cerebellum (C) using Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011), (1:50). BKR1 is expressed in neuronal cells of the amygdala (A) and hippocampus (CA1) (B) as well as in the axons of Purkinje cells in the cerebellum (arrowheads on C). Hoechst 33342 is used as the counterstain.- Mouse spinal cord (1:200), (Dutra, R.C. et al. (2013) Neurobiol. Dis. 54, 82.).
Properties
Sequence
- (C)KEAS RTR*SGGPKGS K, corresponding to amino acid residues 243-257 of rat B1R with replacement of cysteine 250 (C250) with serine (*S) (Accession P97583).
Accession (Uniprot) Number P97583
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
