Overview
Type: Synthetic peptide
Form: Lyophilized powder
B2 Bradykinin Receptor/BDKRB2 Blocking Peptide (#BLP-BR012) is the original antigen used for immunization during Anti-B2 Bradykinin Receptor (BDKRB2) Antibody (#ABR-012) generation. The blocking peptide binds and ‘blocks’ Anti-B2 Bradykinin Receptor/BDKRB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-B2 Bradykinin Receptor (BDKRB2) Antibody (#ABR-012), (1:1000).
2. Anti-B2 Bradykinin Receptor (BDKRB2) Antibody, preincubated with B2 Bradykinin Receptor/BDKRB2 Blocking Peptide (#BLP-BR012).
Expression of B2 bradykinin receptor in mouse spinal cordImmunohistochemical staining of mouse spinal cord using Anti-B2 Bradykinin Receptor (BDKRB2) Antibody (#ABR-012). A. BKR2 (red) appears in several spinal cord neurons (triangles). B. Parvalbumin (green) appears in neurons. C. Merge of BKR2 and parvalbumin shows co-expression in several neurons (triangles) but not all (arrow in C). DAPI is used as the counterstain.
Properties
Sequence
- GKRFRKKSWEVYQG(C), corresponding to amino acid residues 336-349 of human B2R (Accession P30411).
Accession (Uniprot) Number P30411
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
