Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)VLEKVPVSKGQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor) (Accession P23560).
Accession (Uniprot) Number P23560
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis with Anti-BDNF Antibody:1,5. Recombinant human BDNF protein (#B-250).
2,6. Recombinant mouse proBDNF protein (#B-240).
3,7. Recombinant human beta-NGF protein (#N-245).
4,8. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260).
Lanes 1-4: Anti-BDNF Antibody (#ANT-010), (1:200).
Lanes 5-8: Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010).
Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.- Mouse brain sections.
Western blot analysis of 100 ng of each Recombinant human BDNF protein (#B-250) (lanes 1 and 5), Recombinant mouse proBDNF protein (#B-240) (lanes 2 and 6), Recombinant human Neurotrophin-3 (NT-3) protein (#N-260) (lanes 3 and 7) and Recombinant human beta-NGF protein (#N-245) (lanes 4 and 8):1-4. Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:500).
5-8. Guinea pig Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010).
Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.- Mouse and rat brain sections (1:300-1:400).
Last update: 02/01/2024
