Overview
Type: Synthetic peptide
Form: Lyophilized powder
Bestrophin-1 (extracellular) Blocking Peptide (#BLP-BC001) is the original antigen used for immunization during Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Bestrophin-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 3) and rat eye (lanes 2 and 4) lysate:1,2. Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001), (1:200).
3,4. Anti-Bestrophin-1 (extracellular) Antibody, preincubated with Bestrophin-1 (extracellular) Blocking Peptide (#BLP-BC001).
Expression of Bestrophin-1 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001), (1:100). BEST1 is expressed both in the respiratory epithelium (red arrows) and in vascular smooth muscle (black arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)NPNKDYPGHEMD, corresponding to amino acid residues 259-270 of mouse Bestrophin-1 (Accession O88870).
Accession (Uniprot) Number O88870
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
