Overview
Type: Synthetic peptide
Form: Lyophilized powder
Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002) is the original antigen used for immunization during Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002) generation. The blocking peptide binds and ‘blocks’ Anti-Bestrophin-2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat colon membranes:1. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:200).
2. Anti-Bestrophin-2 (extracellular) Antibody, preincubated with Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002).
Western blot analysis of human colon cancer cell lines Colo-205 (lanes 1 and 4), human T-84 colonic adenocarcinoma cell line (lanes 2 and 5) and human colon cancer HT-29 cell line (lanes 3 and 6):1-3. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:200).
4-6. Anti-Bestrophin-2 (extracellular) Antibody, preincubated with Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002).
Expression of Bestrophin-2 in rat colonImmunohistochemical staining of rat colon paraffin embedded sections using Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:100), (brown). BEST2 is expressed in the columnar absorptive cells of the colonic epithelium (arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2 (Accession Q8BGM5).
Accession (Uniprot) Number Q8BGM5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
