Overview
Type: Synthetic peptide
Form: Lyophilized powder
Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004) is the original antigen used for immunization during Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Bombesin Receptor 1/NMBR primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).
3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody, preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).
Western blot analysis of human lung carcinoma NCI-H526 (lanes 1 and 3) and human prostate carcinoma PC-3 (lanes 2 and 4) cell line lysates:1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).
3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody, preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).
Expression of Bombesin receptor 1 in rat hypothalamusImmunohistochemical staining of frozen rat hypothalamus sections using Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:200). A. Staining (red) appears in the neuropil near the ventricle (asterisk) and in neurons (triangles). Calbindin D28k staining (green) appears in neurons. C. Merge of (A) and (B) shows co-expression of BB1R and Calbindin in a few neurons (vertical arrow). DAPI is used as the counterstain.
Properties
Sequence
- (C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1R (Accession P28336).
Accession (Uniprot) Number P28336
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
