Overview
Type: Synthetic peptide
Form: Lyophilized powder
Bombesin Receptor 3 (extracellular) Blocking Peptide (#BLP-BR003) is the original antigen used for immunization during Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Bombesin Receptor 3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003), (1:200).
2. Anti-Bombesin Receptor 3 (extracellular) Antibody, preincubated with Bombesin Receptor 3 (extracellular) Blocking Peptide (#BLP-BR003).
Western blot analysis of mouse brain lysate:1. Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003), (1:200).
2. Anti-Bombesin Receptor 3 (extracellular) Antibody, preincubated with Bombesin Receptor 3 (extracellular) Blocking Peptide (#BLP-BR003).
Western blot analysis of human malignant melanoma cell line Malme-3M (lanes 1 and 3) and human normal skin fibroblast cell line Malme-3 (lanes 2 and 4):1,2. Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003), (1:500).
3,4. Anti-Bombesin Receptor 3 (extracellular) Antibody, preincubated with Bombesin Receptor 3 (extracellular) Blocking Peptide (#BLP-BR003).
Expression of BRS3 in rat testesImmunohistochemical staining of paraffin embedded rat testes section using Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003), (1:50). The area of the efferent ductules near the epididymis is shown. Intense stain (brown) is specific for the pseudostratified epithelium of the efferent ductules. DAB is used for the color reaction. H&E is used as the counterstain.
Properties
Sequence
- (C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BRS3 (Accession P32247).
Accession (Uniprot) Number P32247
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
