Overview
Type: Synthetic peptide
Form: Lyophilized Powder
C3aR1 (extracellular) Blocking Peptide (#BLP-AR036) is the original antigen used for immunization during Anti-C3aR1 (extracellular) Antibody (#AAR-036) generation. The blocking peptide binds and ‘blocks’ Anti-C3aR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-C3aR1 (extracellular) Antibody (#AAR-036), (1:200).
3-4. Anti-C3aR1 (extracellular) Antibody, preincubated with C3aR1 (extracellular) Blocking Peptide (BLP-AR036).
Western blot analysis of mouse M1 myeloid leukemia cell line lysate (lanes 1 and 3) and mouse J774 macrophage cell line lysate (lanes 2 and 4):1-2. Anti-C3aR1 (extracellular) Antibody (#AAR-036), (1:200).
3-4. Anti-C3aR1 (extracellular) Antibody, preincubated with C3aR1 (extracellular) Blocking Peptide (BLP-AR036).
Expression of C3aR1 in mouse hypothalamus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-C3aR1 (extracellular) Antibody (#AAR-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventromedial hypothalamus shows immunoreactivity (green) in neuronal outlines (arrows). B. Pre-incubation of the antibody with C3aR1 (extracellular) Blocking Peptide (BLP-AR036), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
Expression of C3aR1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-C3aR1 (extracellular) Antibody (#AAR-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. C3aR1 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with C3aR1 (extracellular) Blocking Peptide (BLP-AR036), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CRYNFDSSRSYDYWD, corresponding to amino acid residues 172 - 186 of mouse C3aR1(Accession O09047).
Accession (Uniprot) Number O09047
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
