Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNA1A/Cav2.1 Blocking Peptide (#BLP-CC001) is the original antigen used for immunization during Anti-CACNA1A (CaV2.1) Antibody (#ACC-001) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA1A/Cav2.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), (1:200).
2. Anti-CACNA1A (CaV2.1) Antibody, preincubated with CACNA1A/Cav2.1 Blocking Peptide (#BLP-CC001).
Expression of CACNA1A in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), (1:100). A. CACNA1A channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. C. Merged image of panels A and B.
Properties
Sequence
- (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CACNA1A (Accession P54282).
Accession (Uniprot) Number P54282
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 10 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
