Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNA1B/Cav2.2 Blocking Peptide (#BLP-CC002) is the original antigen used for immunization during Anti-CACNA1B (CaV2.2) Antibody (#ACC-002) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA1B/Cav2.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:200).
2. Anti-CACNA1B (CaV2.2) Antibody, preincubated with the control antigen.
Expression of CaV2.2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:100). A. CaV2.2 (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje cells with mouse anti-Calbindin 28K (green) demonstrates the restriction of CaV2.2 to cell bodies but not to dendrites in the molecular layer. C. Merged image of panels A and B.
Properties
Sequence
- (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat CACNA1B (Accession Q02294).
Accession (Uniprot) Number Q02294
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
