Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNA1G/Cav3.1 Blocking Peptide (#BLP-CC021) is the original antigen used for immunization during Anti-CACNA1G (CaV3.1) Antibody (#ACC-021) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA1G/Cav3.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CACNA1G (CaV3.1) Antibody (#ACC-021), (1:200).
2. Anti-CACNA1G (CaV3.1) Antibody, preincubated with CACNA1G/Cav3.1 Blocking Peptide (#BLP-CC021).
Expression of CACNA1G in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-CACNA1G (CaV3.1) Antibody (#ACC-021). A. CACNA1G immunoreactivity (green) appears in the molecular layer. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images A and B. Mol = molecular layer.- Human myometrium (1:200) (Blanks, A.M. et al. (2007) J. Physiol. 581, 915.).
Properties
Sequence
- MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G (Accession O54898).
Accession (Uniprot) Number O54898
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
