Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103) is the original antigen used for immunization during Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA2D3/Cavα2δ3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and human K562 chronic myelogeneous leukemia (lanes 2 and 4) lysates:1,2. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103), (1:200).
3,4. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody, preincubated with CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103).
Expression of CaVα2δ3 in rat hippocampus and cortexImmunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103). In both areas CaVα2δ3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).
Properties
Sequence
- CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaVα2δ3 (Accession Q8CFG5).
Accession (Uniprot) Number Q8CFG5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
