Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNA2D4/Cavα2δ4 (extracellular) Blocking Peptide (#BLP-CC104) is the original antigen used for immunization during Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA2D4/Cavα2δ4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1 and 3) and rat PC12 pheochromocytoma cell lysates (lanes 2 and 4):1,2. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:200).
3,4. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody, preincubated with CACNA2D4/Cavα2δ4 (extracellular) Blocking Peptide (#BLP-CC104).
Expression of CaVα2δ4 in rat DRGImmunohistochemical staining of rat DRG frozen section using Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:100). A. CaVα2δ4 is expressed in DRG neurons. B. Nuclear staining using Hoechst 33342 as the counterstain. C. Merged images of A and B.
Properties
Sequence
- (C)SERPQEMGRLLGEADG, corresponding to amino acid residues 881-896 of mouse CaVα2δ4 (Accession Q5RJF7).
Accession (Uniprot) Number Q5RJF7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
