Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNB1 Blocking Peptide (#BLP-CC106) is the original antigen used for immunization during Anti-CACNB1 Antibody (#ACC-106) generation. The blocking peptide binds and ‘blocks’ Anti-CACNB1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat cortex (lane 3 and 6):1-3. Anti-CACNB1 Antibody (#ACC-106), (1:800).
4-6. Anti-CACNB1 Antibody, preincubated with CACNB1 Blocking Peptide (#BLP-CC106).
Expression of CACNB1 (CaVβ1) in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CACNB1 Antibody (#ACC-106). A. CaVβ1 staining (red) appears in dendrites of the stratum radiatum (arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge image of A and B.
Properties
Sequence
- (C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat CACNB1 (Accession P54283).
Accession (Uniprot) Number P54283
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
