Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNG1 (extracellular) Blocking Peptide (#BLP-CC111) is the original antigen used for immunization during Anti-CACNG1 (extracellular) Antibody (#ACC-111) generation. The blocking peptide binds and ‘blocks’ Anti-CACNG1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat skeletal muscle lysate:1. Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:400).
2. Anti-CACNG1 (extracellular) Antibody, preincubated with CACNG1 (extracellular) Blocking Peptide (#BLP-CC111).
Expression of CaVγ1 (CACNG1) in rat skeletal muscleImmunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:100), followed by DAB. CaVγ1 staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)DHWAVLSPHLEHHNE, corresponding to amino acid residues 30-44 of rat CACNG1 (Accession P97707).
Accession (Uniprot) Number P97707
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
