Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNG6 (extracellular) Blocking Peptide (#BLP-CC112) is the original antigen used for immunization during Anti-CACNG6 (extracellular) Antibody (#ACC-112) generation. The blocking peptide binds and ‘blocks’ Anti-CACNG6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat skeletal muscle lysate:1. Anti-CACNG6 (extracellular) Antibody (#ACC-112), (1:200).
2. Anti-CACNG6 (extracellular) Antibody, preincubated with CACNG6 (extracellular) Blocking Peptide (#BLP-CC112).
Expression of CaVγ6 (CACNG6) in rat brainImmunohistochemical staining of rat cingulate cortex using Anti-CACNG6 (extracellular) Antibody (#ACC-112), (1:400). A. CaVγ6 staining (green) appears in the pyramidal layer (arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
Properties
Sequence
- CIKRLWQADVPAGR, corresponding to amino acid residues 87-100 of rat CACNG6 (Accession Q8VHW3).
Accession (Uniprot) Number Q8VHW3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
