Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNG7 (extracellular) Blocking Peptide (#BLP-CC109) is the original antigen used for immunization during Anti-CACNG7 (extracellular) Antibody (#ACC-109) generation. The blocking peptide binds and ‘blocks’ Anti-CACNG7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-CACNG7 (extracellular) Antibody (#ACC-109), (1:1000).
3,4. Anti-CACNG7 (extracellular) Antibody, preincubated with CACNG7 (extracellular) Blocking Peptide (#BLP-CC109).
Expression of CaVγ7 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CACNG7 (extracellular) Antibody (#ACC-109), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CaVγ7 immunoreactivity (green) appears in in Purkinje cells (arrows). B. Pre-incubation of the antibody with CACNG7 (extracellular) Blocking Peptide (#BLP-CC109), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)REKGR(S)VASEYFLE, corresponding to amino acid residues 67-80 of human CACNG7 (Accession P62955).
Accession (Uniprot) Number P62955
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
