Overview
Type: Synthetic peptide
Form: Lyophilized powder
Calcium Sensing Receptor (extracellular) Blocking Peptide (#BLP-CR004) is the original antigen used for immunization during Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Calcium Sensing Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Colo205 (lanes 1 and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates:1,2,5. Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004), (1:200).
3,4,6. Anti-Calcium Sensing Receptor (extracellular) Antibody, preincubated with Calcium Sensing Receptor (extracellular) Blocking Peptide (#BLP-CR004).
Expression of CaSR in rat brainImmunohistochemical staining of rat hippocampal CA1 region using Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004). A. CaSR staining appears in the soma and appical dendrites of CA1 pyramidal neurons (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merge image of A and B pannels
Properties
Sequence
- (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR (Accession P41180).
Accession (Uniprot) Number P41180
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
