Overview
Type: Synthetic peptide
Form: Lyophilized powder
CALHM1 Blocking Peptide (#BLP-CC101) is the original antigen used for immunization during Anti-CALHM1 Antibody (#ACC-101) generation. The blocking peptide binds and ‘blocks’ Anti-CALHM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human U-87 MG brain glioblastoma cell line (lanes 3 and 7) and rat C6 brain glioma cell line (lanes 4 and 8) lysates:1-4. Anti-CALHM1 Antibody (#ACC-101), (1:200).
5-8. Anti-CALHM1 Antibody, preincubated with CALHM1 Blocking Peptide (#BLP-CC101).
Expression of CALHM1 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CALHM1 Antibody (#ACC-101). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer (green). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge of A and B.
Properties
Sequence
- (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1 (Accession Q8IU99).
Accession (Uniprot) Number Q8IU99
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
