Cat #: BLP-CC101
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1 (Accession Q8IU99).
Accession (Uniprot) Number Q8IU99
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human U-87 MG brain glioblastoma cell line (lanes 3 and 7) and rat C6 brain glioma cell line (lanes 4 and 8) lysates:1-4. Anti-CALHM1 Antibody (#ACC-101), (1:200).
5-8. Anti-CALHM1 Antibody, preincubated with CALHM1 Blocking Peptide (#BLP-CC101). - Rat hippocampus.
Last update: 02/01/2024