Overview
Type: Synthetic peptide
Form: Lyophilized powder
Calnexin Blocking Peptide (#BLP-CS009) is the original antigen used for immunization during Anti-Calnexin Antibody (#ACS-009) generation. The blocking peptide binds and ‘blocks’ Anti-Calnexin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membrane (lanes 1 and 5), mouse heart membrane (lanes 2 and 6), human breast adenocarcinoma (MCF7) lysate (lanes 3 and 7) and human ovary adenocarcinoma (OVCAR-3) cell line lysate (lanes 4 and 8):1-4. Anti-Calnexin Antibody (#ACS-009), (1:400).
5-8. Anti-Calnexin Antibody, preincubated with Calnexin Blocking Peptide (#BLP-CS009).
Expression of Calnexin in BV-2 cells (lane 1) and in BV-2-derived exosomes (lane 2).Western blot was done using Anti-Calnexin Antibody (#ACS-009), (1:400).
Note: Calnexin is a negative control marker of exosomes. It is used to assess the purity of exosome isolation.
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lane 1) and U-87 MG-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-Calnexin Antibody (#ACS-009), (1:600).
Note: Calnexin is a negative control marker of exosomes. It is used to assess the purity of exosome isolation.
Expression of Calnexin in mouse hippocampusImmunohistochemical staining of mouse hippocampal CA1 region using Anti-Calnexin Antibody (#ACS-009), (1:200). A. Canx staining (green) appears in neurons (horizontal arrow) and astrocytes (vertical arrow). B. GFAP staining of astrocytic fibers (red). C. Merge of panels A and B demonstrates co-localization in some astrocytes. DAPI is used to stain nuclei (blue).
Properties
Sequence
- (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx (Accession P35565).
Accession (Uniprot) Number P35565
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
