Overview
Lyophilized Powder yes
Origin Discodermia calyx (marine sponge).
Source Natural
MW: 1009.2
Purity: >98% (HPLC)
Effective concentration 10-100 nM.
Structure
Chemical name N-[(3S)-[4-(1E)-3-[(2R,3R,4R,7S,8S,9R)-2-[(1S,3S,4S,5R, 7E,9E,11E,13Z)-14-Cyano-3,5-dihydroxy-1-methoxy-4,6,8,9 ,13-pentamethyl-7,9,11,13-tetradecatetraenyl]-9-hydroxy -4,4,8-trimethyl-3-(phosphonooxy)-1,6-dioxaspiro[4.5]de c-7-yl]-1-propenyl]-2-oxazoly]butyl]-4-deoxy-4-(dimethy lamino)-5-O-methyl-L-ribonamide.
Molecular formula C50H81N4O15P.
CAS No.: 101932-71-2
Activity Calyculin A is a very potent, cell-permeable PP-1 and PP-2 inhibitor (IC50 = 2 nM and 0.5-1 nM respectively)1.
References-Activity
- Bechem, M. and Hoffman, H. (1993) Pflugers Arch. 424, 343.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO, ethanol, acetone, or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
Protect from light.
Protect from light.
Our bioassay
Alomone Labs Calyculin A inhibits the growth of breast cancer epithelial MCF7 cells.Cells were incubated with different concentrations of Calyculin A (#C-100) for four days. Cell number was measured using the methylene blue method. The number of cells was normalized with respect to the control (100%) and plotted against Calyculin A concentrations.
References - Scientific background
- Kateo, Y. et. al. (1996) J. Am. Chem. Soc. 108, 8712178.
- Bechem, M. and Hoffman, H. (1993) Pflugers Arch. 424, 343.
- Hecheler, J. et al. (1988) Pflugers Arch. 412, 248.
Scientific background Calyculin A was initially isolated from the marine sponge Discodermia calyx.1 It is a very potent, cell-permeable PP-1 and PP-2 inhibitor (IC50 2nM and 0.5-1nM respectively),2 and extremely specific for PP-1 and PP-2A among other acidic, alkaline and protein phosphatases.2,3
Lyophilized Powder
Calyculin A (#C-100) is a highly pure, natural, and biologically active compound.
For research purposes only, not for human use
Last Update: 07/05/2024
Applications
Citations
Citations
Powered by BiozProduct citations
- Fournier, C. et al. (2012) Sci. Rep. 2, 643.
