Overview
Structure
Scientific background
Camptothecin is an anti-cancer alkaloid that specifically inhibits DNA topoisomerase I and DNA topoisomerase II (topoisomerase I/II).1-3 The highly conserved region around Gly505 of topoisomerase I was found to be important for camptothecin activity.4
Camptothecin converts topoisomerase into a cell poison by trapping the cleavable covalent complex of enzyme with duplex DNA and blocking the re-ligation step. Thereby enhancing and stabilizing the formation of DNA breakage which is responsible for cell death.1-9
Camptothecin inhibits (IC50 = 30 nM) Tat protein-mediated transactivation of HIV-1 and enhances PKCa activity during apoptosis in HL60 myeloid leukemia cells via a caspase-regulated mechanism.9,10
For research purposes only, not for human use
Specifications
Applications
Our Bioassay
Our bioassay
Alomone Labs Camptothecin inhibits the proliferation of MC-7 breast cancer cells.Cells were treated with different concentrations of Camptothecin (#C-330) for 4 days. The number of live cells was measured by XTT cell proliferation assay kit, normalized to the control (100%) and plotted against the drug concentration.
Last Update: 08/01/2025
