Cat #: AK-330_KIT
Size: 13 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-Kir2.1/KCNJ2 Antibody Cat #: APC-026
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1 (Accession P48049). Intracellular, C-terminal part.
Homology
Rabbit, bovine, pig, guinea pig - identical; rat, mouse - 17/19 amino acid residues identical; chicken - 15/19 amino acid residues identical.
Kir2.1/KCNJ2 Blocking Peptide Cat #: BLP-PC026
Size:
40 µg
Guinea pig Anti-Kir2.1/KCNJ2 Antibody Cat #: APC-026-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1 (Accession P48049). Intracellular, C-terminus.
Homology
Rabbit, bovine, pig, guinea pig - identical; rat, mouse - 17/19 amino acid residues identical; chicken - 15/19 amino acid residues identical.
Kir2.1/KCNJ2 Blocking Peptide Cat #: BLP-PC026
Size:
40 µg
Anti-Kir2.2 (KCNJ12) Antibody Cat #: APC-042
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.
Homology
Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.
Kir2.2/KCNJ12 Blocking Peptide Cat #: BLP-PC042
Size:
40 µg
Anti-GIRK1 (Kir3.1) Antibody Cat #: APC-005
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence LQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGPTRMEGNLPAKLRKMNSDRFT, corresponding to residues 437-501 of mouse GIRK1 (Accession P63250). Intracellular, C-terminus.
Homology
Rat - identical; human - 64/66 amino acid residues identical; guinea pig - 63/66 amino acid residues identical; chicken - 59/66 amino acid residues identical.
GIRK1/Kir3.1 Blocking Peptide Cat #: BLP-PC005
Size:
0.12 mg
Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody Cat #: ALM-031
Size:
25 µg
Host: Mouse
Type: Monoclonal
Application:
IC, IF, LCI, WB
Reactivity:
H, M
Immunogen
Synthetic peptide mapping to the extracellular loop of human GIRK1 (Accession P48549).
Anti-KCNJ5 (Kir3.4) Antibody Cat #: APC-027
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4 (Accession P48548). Intracellular, N-terminus.
Homology
Mouse, human, pig - identical.
KCNJ5/Kir3.4 Blocking Peptide Cat #: BLP-PC027
Size:
40 µg
Anti-Kir6.2 Antibody Cat #: APC-020
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminal part.
Homology
Mouse - identical; human - 12/14 amino acid residues identical.
Kir6.2 Blocking Peptide Cat #: BLP-PC020
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
A mouse monoclonal antibody (#ALM-031) and guinea pig polyclonal antibody (#APC-026-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
