Overview
Type: Synthetic peptide
Form: Lyophilized powder
Caspr2 Blocking Peptide (#BLP-PZ005) is the original antigen used for immunization during Anti-Caspr2 Antibody (#APZ-005) generation. The blocking peptide binds and ‘blocks’ Anti-Caspr2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Caspr2 Antibody (#APZ-005), (1:200).
3,4. Anti-Caspr2 Antibody, preincubated with Caspr2 Blocking Peptide (#BLP-PZ005).
Expression of Caspr2 in rat cerebellumImmunohistochemical staining of rat brain frozen sections using Anti-Caspr2 Antibody (#APZ-005), (1:200), (green). A. Caspr2 appears in pinceau structures (arrows) and diffusely in the molecular layer (MOL). Staining of the same section for parvalbumin (red) labels Purkinje cells and demonstrates the position of the pinceau under Purkinje cells. B. Pre-incubation of the antibody with the Caspr2 peptide antigen blocks the staining, demonstrating specificity.
Properties
Sequence
- (C)DPNFTETIDESKKEWLI, corresponding to amino acid residues 1315-1331 of human Caspr2 (Accession Q9UHC6).
Accession (Uniprot) Number Q9UHC6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
