Name: Cav1.2/CACNA1C Blocking Peptide
Brand: Alomone Labs
SKU: BLP-CC003

Overview

Cat #: BLP-CC003

Type: Synthetic peptide

Form: Lyophilized powder

Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003) is the original antigen used for immunization during Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.2/CACNA1C primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Certificate of Analysis SDS

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat brain membranes:
1. Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003), (1:200).
2. Anti-Ca_V1.2 (CACNA1C) Antibody, preincubated with Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003).
Western blot analysis of Ca_V1.2-transfected Xenopus oocytes (lane 1) and non-transfected oocytes lysates (lane 2):
1. Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003), (1:200) in Ca_V1.2 (CACNA1C) Channel Overexpressed in Xenopus oocytes.
2. Anti-Ca_V1.2 (CACNA1C) Antibody in non-transfected oocytes.
Expression of Ca_V1.2 in mouse cerebellum
Immunohistochemical staining of mouse cerebellum with Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003). A. Ca_V1.2 (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows). B. Staining of Purkinje nerve cells with mouse anti-Calbindin 28K (green) demonstrates the location of dendrites in the molecular layer. C. Merged image of panels A and B.
Mouse atrioles (1:300) (Howitt, L. et al. (2013) J. Physiol. 591, 2157.).
Western blot analysis of rat brain membrane:
1. Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP), (1:200).
2. Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody, preincubated with Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003).
Western blot analysis of Ca_V1.2-transfected Xenopus oocytes (lane 1) and non-transfected oocytes lysates (lane 2):
1. Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP), (1:200) in Ca_V1.2 (CACNA1C) Channel Overexpressed in Xenopus oocytes.
2. Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody in non-transfected oocytes.
Multiplex staining of Ca_V1.2 and GABA(A) α1 Receptor in rat cerebellum
Immunohistochemical staining of rat cerebellum using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP) and Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG). A. Ca_V1.2 (red) is detected mostly in Purkinje cells (arrow). B. In the same section, GABA(A) α1 Receptor (green) is observed in the granule layer. C. Merge of the two images suggests some colocalization between Ca_V1.2 and GABA(A) α1 Receptor in the rat granule layer but only Ca_V1.2 appears in Purkinje cells.
Expression of Ca_V1.2 in human atria
Immunohistochemical staining of human left atrium using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP), (1:100).
The picture was kindly provided by Dr. Van Wagoner, D.R. from the Department of Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio, USA. Lovano, B. and Peterson, J. collected the data.
Expression of Ca_V1.2 in rat heart
Immunohistochemical staining of rat heart paraffin embedded sections using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP). A. Ca_V1.2 staining (green) appears mainly in the cardiac muscle, and in a lesser intensity in the tunica intima layer of the smooth muscle of the muscular arteries. B. Nuclear staining using DAPI as the counter stain. C. Merged images of A and B.
Expression of Ca_V1.2 in mouse hippocampus
Immunohistochemical staining of mouse dentate gyrus using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP). A. Ca_V1.2 (green) appeared in the outer molecular layer of the dentate gyrus and in the granule layer. B. Counterstain with DAPI (blue) outlines the granule layer of the dentate gyrus.

Properties

Sequence

(C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2 (Accession P22002).

Accession (Uniprot) Number P22002

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Concentration After Reconstitution 0.4 mg/ml.

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Specific Use it with:

Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize Ca_V1.2 from mouse, rat, and human samples.

Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003)
Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP, formerly AGP-001), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ca_V1.2 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003-GP)