Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2 (Accession P22002).
Accession (Uniprot) Number P22002
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), (1:200).
2. Anti-CaV1.2 (CACNA1C) Antibody, preincubated with Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003).
Western blot analysis of CaV1.2-transfected Xenopus oocytes (lane 1) and non-transfected oocytes lysates (lane 2):1. Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), (1:200) in CaV1.2 (CACNA1C) Channel Overexpressed in Xenopus oocytes.
2. Anti-CaV1.2 (CACNA1C) Antibody in non-transfected oocytes.- Rat brain sections (see also 3, 5, 7, 10 of recent publications using this product). Mouse atrioles (1:300) (Howitt, L. et al. (2013) J. Physiol. 591, 2157.).
Western blot analysis of rat brain membrane:1. Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP), (1:200).
2. Guinea pig Anti-CaV1.2 (CACNA1C) Antibody, preincubated with Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003).- Rat heart and pancreas and cerebellum (1:100). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
