Overview
Lyophilized Powder yes
Accession number M57974.1
Origin Rabbit CaV1.2 cDNA (M57974.1) was transcribed in vitro to produce full length mRNA. CaV1.2 mRNA was injected into Xenopus oocytes and protein expression was verified by detecting voltage dependent Nifedipine (#N-120) sensitive ionic current. Membrane fraction was prepared from oocyte population homogenate and lyophilized. Non-injected oocytes underwent the same procedure excluding mRNA injection.
Storage before reconstitution Lyophilized injected and non-injected membrane fractions can be stored at room temperature for 2 weeks. For longer periods, they should be stored at -20°C.
Reconstitution Work on ice. Gently reconstitute injected and non-injected membrane fractions in 100 µl DDW (total protein concentration is 0.3 µg/µl). Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution Up to 2 weeks at -20°C. Avoid repeated thawing and freezing.
Western blot
Western blot analysis of CaV1.2 (CACNA1C) Channel Overexpressed Membrane Fractions (#LX-104) obtained from Xenopus oocyte membrane lysates expressing rabbit CaV1.2 channels (lanes 2, 4 and 6), and non-injected oocyte lysates (lanes 1, 3 and 5):1,2. Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#AGP-001), (1:200).
3,4. Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), (1:200).
5,6. Anti-CaV1.2a (CACNA1C) Antibody (#ACC-013), (1:200).
Note
Lyophilized membrane fraction was reconstituted in 100 μl DDW (see below). 100 μl of Sample Buffer X2 was added (total protein concentration was 0.15 μg/μl). Heated samples (70°C) were loaded onto 10% acrylamide gel (10-40 μl/lane) and resolved using SDS-PAGE gel, followed by western blot analysis using anti-CaV1.2 antibodies (Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#AGP-001) and Anti-CaV1.2a (CACNA1C) Antibody (#ACC-013)) following product instructions.
This product includes:
1 x 0.1 ml lyophilized CaV1.2 channel overexpressed membrane lysate
1 x 0.1 ml lyophilized non-injected Xenopus oocytes membrane lysate
Lyophilized Powder
For research purposes only, not for human use
Last Update: 31/12/2019
Specifications
Citations
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