Overview
Type: Synthetic peptide
Form: Lyophilized powder
Cav1.3/CACNA1D (extracellular) Blocking Peptide (#BLP-CC311) is the original antigen used for immunization during Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.3/CACNA1D (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), and rat C6 brain Glioma (lanes 3 and 6) lysates:1-3. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:200).
4-6. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody, preincubated with Cav1.3/CACNA1D (extracellular) Blocking Peptide (#BLP-CC311).
Expression of CaV1.3 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311). A. CaV1.3 labeling (red) appears in the cell bodies of the DRG. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Properties
Sequence
- (C)EQLTKETEGGNHS, corresponding to amino acid residues 215-227 of rat CaV1.3 (Accession P27732).
Accession (Uniprot) Number P27732
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
