Cat #: BLP-CC025
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of rat CaV3.2 (Accession Q9EQ60).
Accession (Uniprot) Number Q9EQ60
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat DRG lysates:1. Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:200).
2. Anti-CaV3.2 (CACNA1H) Antibody, preincubated with Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025). - Mouse cerebellum (1:100), rat DRG (1:50).
Last update: 02/01/2024