Overview
Type: Synthetic peptide
Form: Lyophilized powder
Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009) is the original antigen used for immunization during Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009) generation. The blocking peptide binds and ‘blocks’ Anti-Cav3.3/CACNA1I primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400).
3-4. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).- Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).
Expression of CaV3.3 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400), followed by donkey anti-rabbit-Cy3. A. Staining in the hippocampal CA3 region, showed Cav3.3 immunoreactivity (red) appeared in soma of pyramidal neuron profiles (horizontal arrows) and in dendrites (vertical arrows) projecting into the stratum radiatum (SR). B. Pre-incubation of the antibody with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.
Properties
Sequence
- CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3 (Accession Q9Z0Y8).
Accession (Uniprot) Number Q9Z0Y8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
