Overview
Cat #: BLP-CR042
Type: Synthetic peptide
Form: Lyophilized powder
CCKBR (extracellular) Blocking Peptide (#BLP-CR042) is the original antigen used for immunization during Anti-CCKBR (extracellular) Antibody (#ACR-042) generation. The blocking peptide binds and ‘blocks’ Anti-CCKBR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200).
3,4. Anti-CCKBR (extracellular) Antibody, preincubated with CCKBR (extracellular) Blocking Peptide (#BLP-CR042). - Expression of Cholecystokinin B receptor in rat stomachImmunohistochemical staining of paraffin embedded rat stomach sections using Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:100). Cholecystokinin B receptor (brown) is expressed in both parietal cells (black arrows) and in chief cells (red arrows) of the gastric mucosa. Hematoxilin is used as the counterstain.
- Expression of CCKBR in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat dorsomedial hypothalamus region, showed immunoreactivity (green) in neurons (vertical arrows) and along the wall of 3rd ventricle (horizontal arrow). B. Pre-incubation of the antibody with CCKBR (extracellular) Blocking Peptide (#BLP-CR042), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.
- Expression of CCKBR in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule layer (G, vertical arrows) and in interneurons (horizontal arrows). B. Pre-incubation of the antibody with CCKBR (extracellular) Blocking Peptide (#BLP-CR042), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CETPRIRGTGTRELE, corresponding to amino acid residues 39-53 of mouse Gastrin/cholecystokinin type B receptor (Accession P56481).
Accession (Uniprot) Number P56481
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.