Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CCR2 (extracellular) Blocking Peptide (#BLP-CR072) is the original antigen used for immunization during Anti-CCR2 (extracellular) Antibody (#ACR-072) generation. The blocking peptide binds and ‘blocks’ Anti-CCR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and mouse spleen membranes (lanes 3 and 6):1-3. Anti-CCR2 (extracellular) Antibody (#ACR-072), (1:200).
4-6. Anti-CCR2 (extracellular) Antibody, preincubated with CCR2 (extracellular) Blocking Peptide (BLP-CR072).
Expression of CCR2 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CCR2 (extracellular) Antibody (#ACR-072), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CCR2 immunoreactivity (green) appears in the pyramidal layer (P) and in apical dendrites (arrows) in the hippocampal CA1 region. B. Pre-incubation of the antibody with CCR2 (extracellular) Blocking Peptide (BLP-CR072), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of CCR2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CCR2 (extracellular) Antibody (#ACR-072), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CCR2 immunoreactivity (green) appears in the pyramidal layer (P) and in apical dendrites (arrows) in the hippocampal CA1 region. B. Pre-incubation of the antibody with CCR2 (extracellular) Blocking Peptide (BLP-CR072), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HSLFTRSIQELDEG, corresponding to amino acid residues 19 - 32 of mouse CCR2 (Accession P51683).
Accession (Uniprot) Number P51683
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
