Overview
Type: Synthetic peptide
Form: Lyophilized powder
CCR7 (extracellular) Blocking Peptide (#BLP-CR027) is the original antigen used for immunization during Anti-CCR7 (extracellular) Antibody (#ACR-027) generation. The blocking peptide binds and ‘blocks’ Anti-CCR7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human T-cell leukemia Jurkat cell line (lanes 1 and 4), human acute lymphoblastic leukemia MOLT-4 (lanes 2 and 5) and mouse macrophage J774.1 (lanes 3 and 6) cell lysates:1-3. Anti-CCR7 (extracellular) Antibody (#ACR-027), (1:200).
4-6. Anti-CCR7 (extracellular) Antibody, preincubated with CCR7 (extracellular) Blocking Peptide (#BLP-CR027).
Expression of CCR7 in mouse deep cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CCR7 (extracellular) Antibody (#ACR-027), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. CCR7 immunoreactivity (green) appears in blood vessels (arrows). B. Pre-incubation of the antibody with CCR7 (extracellular) Blocking Peptide (#BLP-CR027), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DEVTDDYIGDNTTVD, corresponding to amino acid residues 26-40 of human CCR7 (Accession P32248).
Accession (Uniprot) Number P32248
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
