Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CD38 (extracellular) Blocking Peptide (#BLP-NT068) is the original antigen used for immunization during Anti-CD38 (extracellular) Antibody (#ANT-068) generation. The blocking peptide binds and ‘blocks’ Anti-CD38 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-CD38 (extracellular) Antibody (#ANT-068), (1:400).
3-4. Anti-CD38 (extracellular) Antibody, preincubated with CD38 (extracellular) Blocking Peptide (BLP-NT068).
Expression of CD38 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CD38 (extracellular) Antibody (#ANT-068), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CD38 immunoreactivity (green), appears in purkinje cells (arrows) and in the molecular layer (M). B. Pre-incubation of the antibody with CD38 (extracellular) Blocking Peptide (BLP-NT068), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, G = granule layer.
Expression of CD38 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CD38 (extracellular) Antibody (#ANT-068), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CD38 immunoreactivity (green), appears in purkinje cells (arrows) and in the molecular layer (M). B. Pre-incubation of the antibody with CD38 (extracellular) Blocking Peptide (BLP-NT068), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, G = granule layer.
Properties
Sequence
- Peptide (C)KSKHLAHQYTWIQGK, corresponding to amino acid residues 131 - 145 of mouse CD38 (Accession P56528).
Accession (Uniprot) Number P56528
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
