Overview
Type: Synthetic peptide
Form: Lyophilized powder
CD56/NCAM1 (extracellular) Blocking Peptide (#BLP-NR041) is the original antigen used for immunization during Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) generation. The blocking peptide binds and ‘blocks’ Anti-CD56/NCAM1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 6), rat cerebellum (lanes 2 and 7), rat eye (lanes 3 and 8), human SH-SY5Y brain neuroblastoma (lanes 4 and 9) and PC12 rat pheochromocytoma (lanes 5 and 10) cells lysates:1-5. Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (lanes 1-3 1:400 and lanes 4-5 1:200).
6-10. Anti-CD56/NCAM1 (extracellular) Antibody, preincubated with CD56/NCAM1 (extracellular) Blocking Peptide (#BLP-NR041).
Expression of NCAM1 in rat olfactory bulbImmunohistochemical staining of rat olfactory bulb frozen section using Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (1:300). A. NCAM1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). B. Nuclei are stained using DAPI as the counterstain. C. Merged image of A and B panels.
Properties
Sequence
- (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1 (Accession P13596).
Accession (Uniprot) Number P13596
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
