Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73 (Accession Q61503).
Accession (Uniprot) Number Q61503
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).
3,4. Anti-CD73 (extracellular) Antibody, preincubated with CD73 (extracellular) Blocking Peptide (#BLP-NT066).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).
3,4. Anti-CD73 (extracellular) Antibody, preincubated with CD73 (extracellular) Blocking Peptide (#BLP-NT066).- Mouse brain sections (1:200).
Last update: 02/01/2024
