Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)EVIKELQEFYKDTYQK, corresponding to amino acid residues 114 - 129 of mouse CD9 (Accession P40240).
Accession (Uniprot) Number P40240
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
3-4. Anti-CD9 (extracellular) Antibody, preincubated with CD9 (extracellular) Blocking Peptide (BLP-NR209).
Western blot analysis of rat lung lysate:1. Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
2. Anti-CD9 (extracellular) Antibody, preincubated with CD9 (extracellular) Blocking Peptide (BLP-NR209).
Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 4), human MEG-01 megakaryoblast cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
4-6. Anti-CD9 (extracellular) Antibody, preincubated with CD9 (extracellular) Blocking Peptide (BLP-NR209).
Western blot analysis of mouse BV-2 cell line lysate (lane 1) and BV-2-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lane 1) and U-87 MG-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
Last update: 16/04/2024
