Overview
Type: Synthetic peptide
Form: Lyophilized powder
CDNF Blocking Peptide (#BLP-NT027) is the original antigen used for immunization during Anti-CDNF Antibody (#ANT-027) generation. The blocking peptide binds and ‘blocks’ Anti-CDNF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of 100 ng human recombinant CDNF (lanes 1 and 3) and 100 ng human recombinant MANF (lanes 2 and 4):1,2. Anti-CDNF Antibody (#ANT-027) (1:500).
3,4. Anti-CDNF Antibody, preincubated with CDNF Blocking Peptide (#BLP-NT027).
Note that the antibody recognizes CDNF but fails to recognize closely related MANF.
Western blot analysis of mouse brain lysate:1. Anti-CDNF Antibody (#ANT-027), (1:200).
2. Anti-CDNF Antibody, preincubated with CDNF Blocking Peptide (#BLP-NT027).
Expression of CDNF in mouse brainImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using rabbit Anti-CDNF Antibody (#ANT-027), (1:300). A. CDNF staining (green) is detected in several cells in the vicinity of the substantia nigra pars compacta. B. Tyrosine hydroxylase staining (red) appears in the substantia nigra. C. Merge of the two images demonstrates dopaminergic (TH-positive) neurons also expressing CDNF (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF (Accession Q49AH0).
Accession (Uniprot) Number Q49AH0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
