Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF (Accession Q49AH0).
Accession (Uniprot) Number Q49AH0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of 100 ng human recombinant CDNF (lanes 1 and 3) and 100 ng human recombinant MANF (lanes 2 and 4):1,2. Anti-CDNF Antibody (#ANT-027) (1:500).
3,4. Anti-CDNF Antibody, preincubated with CDNF Blocking Peptide (#BLP-NT027).
Note that the antibody recognizes CDNF but fails to recognize closely related MANF.
Western blot analysis of mouse brain lysate:1. Anti-CDNF Antibody (#ANT-027), (1:200).
2. Anti-CDNF Antibody, preincubated with CDNF Blocking Peptide (#BLP-NT027).- Mouse brain sections (1:300).
Last update: 02/01/2024
