Overview
Type: Synthetic peptide
Form: Lyophilized powder
CHRM1 (443-458) Blocking Peptide (#BLP-MR010) is the original antigen used for immunization during Anti-CHRM1 (443-458) Antibody (#AMR-010) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM1 (443-458) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:200).
2. Anti-CHRM1 (443-458) Antibody, preincubated with CHRM1 (443-458) Blocking Peptide (#BLP-MR010).
Expression of Muscarinic acetylcholine receptor M1 in mouse striatumImmunohistochemical staining of mouse striatum using Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:100). M1 mAChR staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 muscarinic receptor (Accession P11229).
Accession (Uniprot) Number P11229
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
